Prlhr
Prolactin-releasing peptide receptor (PrRP receptor) (PrRPR) (G protein-coupled receptor 10)
Spatial tier: STRICT_EXCLUSIVE · Class: GPCR · Top region: PMv (Ventral premammillary nucleus) · Positive regions: 11/554
46.9Discovery Score
34.4Targetability Score
CEvidence grade C
What this means: Prlhr: predicted fine-region profile is strict-exclusive, with 11 positive regions out of 554 (limit 12); direct spatial validation is required.
Function in PMv: evidence, prediction, innovation
- Gene function
- Prlhr encodes GPR10, the sole high-affinity Gq/Gi-coupled receptor for prolactin-releasing peptide (PrRP, from Prlh), mobilizing intracellular Ca2+. Central signalling curbs food intake and raises energy expenditure; Prlhr-null mice are obese via reduced expenditure, and GPR10 relays leptin-driven thermogenesis from DMH PrRP neurons.
- Region function
- The ventral premammillary nucleus is a small glutamatergic, LepRb-rich nucleus linking metabolic and prolactin status to GnRH/LH secretion, puberty and odour-evoked behaviour.
- Published in this region
- inferred No region-specific literature found. Closest: Roland et al. 1999's ISH map put GPR10 mRNA in reticular thalamus, periventricular and dorsomedial hypothalamus, NTS and area postrema but not premammillary; PrRP-GPR10 function is dissected only in DMH, PVN/SON and brainstem (Gu et al. 2004; Dodd et al. 2014), PMv being defined by LepRb and PACAP neurons (Donato et al. 2011; Ross et al. 2018).
- Predicted function
- Prediction: GPR10 on PMv LepRb glutamatergic neurons is a second peptidergic metabolic input converging with leptin, depolarizing them via Gq/PLC to reinforce drive to GnRH neurons. PMv-restricted Prlhr deletion should delay puberty and blunt the fasting-refeeding LH rebound without changing body weight.
- Innovation
- ★★★★★ 5/5 GPR10 pharmacology and PMv reproductive physiology are mature, yet this receptor has never been examined in this nucleus.
- Tools
- No validated Prlhr-Cre; Prlh-Cre and Prlh/Prlhr knockouts cover ligand and receptor. Brain-penetrant PrRP-31, PrRP-20 and lipidated PrRP31 analogues and GPR10 antibodies exist; PMv is targeted with Lepr-Cre, PACAP-Cre, Vglut2-Cre.
- References
Direct evidence located at the region
Allen ISH experiment 74511758, section image 74397096 (section 225): the section that contains the CCF centroid of Ventral premammillary nucleus according to the Allen image-to-atlas alignment (reference_to_image), with a 2 mm window around that point. Left: whole section, red box = window. Middle: ISH signal. Right: Allen's expression-mask view of the same window. open this image in the Allen viewer
Look it up yourself: Allen Mouse Brain ISH for PrlhrAllen reference atlas: PMv (structure 1004)ABC Atlas MERFISH viewer(in the ABC Atlas choose dataset MERFISH-C57BL6J-638850-CCF, colour cells by gene "Prlhr" or by parcellation_substructure "PMv")All genes confined to PMv + 3-D brain
Direct spatial evidence
MERFISH REGION LOCATOR
DIRECT ALLEN ISH
DIRECT ALLEN ISH
Regional expression figure
Predicted WMB × fine-CCF profile; not direct full-transcriptome spatial measurement.
Spatial profile
Evidence and specificity
- Evidence status
- PREDICTED_FINE
- Direct sources
- None; predicted localization only
- Exclusive threshold
- 12 positive regions out of 554
- Spatial specificity
- 0.321
- Top-region fraction
- 0.094
- Filter status
- PASS
Interpretation limits
- Cell-type-to-region projection is imputed expression, not direct spatial measurement.
- Adult reference atlases do not establish stability across age, sex, strain, state, or disease.
- Transcript abundance does not guarantee protein abundance or genetic-tool performance.